rnf2  (Proteintech)

Journal: Stem Cell Reviews and Reports

Article Title: The Zinc-Finger Protein POGZ Associates with Polycomb Repressive Complex 1 to Regulate Bone Morphogenetic Protein Signaling During Neuronal Differentiation

doi: 10.1007/s12015-025-11028-x

Figure Lengend Snippet: Novel POGZ-Containing PRC1 Complex Identified in the Developing Nervous System. (a) Schematic displaying the RING1B IP experiment followed by mass spectrometry in (left to right) mouse embryonic brain, cortical neurons, and neuronal progenitors. (b) RING1B interactors identified in (a) are grouped by their association with specific PRC1 subcomplexes. (c) HA IP of NFH-PCGF1-6 HEK 293 T-REx cell lines. Bound proteins were resolved on SDS-PAGE and detected by immunoblotting for the indicated antigens (IN = Input). (d) FLAG IP of NFH-POGZ and NFH-Control HEK 293 T-REx cells. The bound proteins were resolved on SDS-PAGE and blotted for the indicated PRC1.6 components. (e) PLA experiments showing that POGZ and RING1B co-localize in HeLa cells. Single antibody conditions are shown as negative controls. At least 100 cells were quantified for each condition. Bars indicate the mean values, error bars represent standard errors of the mean, and asterisks indicate statistical significance (Mann-Whitney). Immunoblotting of POGZ and RING1B to demonstrate their expression in HeLa cells. (f) Representative micrographs of the PLA experiment, with scale bars indicating 10 μm

Article Snippet: The antibodies used are RING1B (Bethyl, cat. no. A302-869 A-T) and IgG (Cell Signaling, cat. no. 2729 S).

Techniques: Mass Spectrometry, SDS Page, Western Blot, Control, MANN-WHITNEY, Expressing

Journal: Stem Cell Reviews and Reports

Article Title: The Zinc-Finger Protein POGZ Associates with Polycomb Repressive Complex 1 to Regulate Bone Morphogenetic Protein Signaling During Neuronal Differentiation

doi: 10.1007/s12015-025-11028-x

Figure Lengend Snippet: RING1B Activity Is Essential for POGZ-Mediated Transcriptional Repression. (a) Schematic of the luciferase reporter system used to examine the transcriptional activity of POGZ. (b) Fold change in luciferase activity in cells expressing GAL4 only, GAL4–PCGF4, and GAL4–POGZ after 24-hour doxycycline induction. Each value is the mean of three biological replicates, with error bars representing the standard deviation. *** p < 0.001 determined by one-way ANOVA followed by Tukey’s post hoc test, compared with the GAL4 control. (c) Fold change in luciferase activity in GAL4–POGZ cells upon KD of RING1B. Cells were transfected with control or RING1B siRNAs, and luciferase activity was measured after 24-hour doxycycline induction. Each value is the mean of three biological replicates, with error bars representing the standard deviation. ** p < 0.01 by two-sided t-test, compared with the GAL4 control. (d) Immunoblotting of samples used for luciferase activity reporter assay in (c) to demonstrate NG4-POGZ WT induction and RING1B KD efficiency

Article Snippet: The antibodies used are RING1B (Bethyl, cat. no. A302-869 A-T) and IgG (Cell Signaling, cat. no. 2729 S).

Techniques: Activity Assay, Luciferase, Expressing, Standard Deviation, Control, Transfection, Western Blot, Reporter Assay

Journal: Stem Cell Reviews and Reports

Article Title: The Zinc-Finger Protein POGZ Associates with Polycomb Repressive Complex 1 to Regulate Bone Morphogenetic Protein Signaling During Neuronal Differentiation

doi: 10.1007/s12015-025-11028-x

Figure Lengend Snippet: PRC1.6-POGZ genomic localization within embryonic mouse cortical cells. (a) Heatmap showing enrichment of PRC1.6-POGZ, POGZ & RING1B, POGZ & HP1g, and POGZ only bound peaks centered around TSS (± 3 kb) regions across loci. (b) Pie chart showing the percentage of PRC1.6-POGZ target loci on each genomic region. (c) GO analysis of PRC1.6-POGZ target genes in mouse cortical cells. The x-axis (in logarithmic scale) corresponds to the binomial raw P values. (d) Representative genomic tracks showing the normalized tracks of POGZ, RING1B, and HP1g at the indicated loci

Article Snippet: The antibodies used are RING1B (Bethyl, cat. no. A302-869 A-T) and IgG (Cell Signaling, cat. no. 2729 S).

Techniques: